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Coumarin 6 and 1,6-diphenyl-1,3,5-hexatriene (DPH) as Fluorescent Probes to Monitor Protein Aggregation

Coumarin 6 and 1,6-diphenyl-1,3,5-hexatriene (DPH) as Fluorescent Probes to Monitor Protein Aggregation

Pinakin K Makwana, Prashant N Jethva, Ipsita Roy

Analyst. 2011 May 21;136(10):2161-7.

PMID: 21445407

Abstract:

We report the use of Coumarin 6 and 1,6-diphenyl-1,3,5-hexatriene (DPH) for the identification of protein aggregates for the first time. The two dyes can be used at very low (nanomolar) concentrations and do not interfere with the aggregation process, as is reported for other commonly used fluorescent protein probes. In the presence of protein aggregates, their quantum yields are significantly high. DPH is able to recognize both amorphous and fibrillar aggregates but cannot distinguish between them. Coumarin 6 can distinguish between both types of aggregates. It also exhibits the characteristic sigmoidal curve of amyloid formation, with higher sensitivity for detection of fibrillation than the conventionally used Thioflavin T.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP38215360	Coumarin 6		38215-36-0	Price

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