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Covalent Enzyme Immobilization by Poly(ethylene Glycol) Diglycidyl Ether (PEGDE) for Microelectrode Biosensor Preparation

Covalent Enzyme Immobilization by Poly(ethylene Glycol) Diglycidyl Ether (PEGDE) for Microelectrode Biosensor Preparation

Natalia Vasylieva, Bogdan Barnych, Anne Meiller, Caroline Maucler, Loredano Pollegioni, Jian-Sheng Lin, Daniel Barbier, Stéphane Marinesco

Biosens Bioelectron. 2011 Jun 15;26(10):3993-4000.

PMID: 21546239

Abstract:

Poly(ethylene glycol) diglycidyl ether (PEGDE) is widely used as an additive for cross-linking polymers bearing amine, hydroxyl, or carboxyl groups. However, the idea of using PEGDE alone for immobilizing proteins on biosensors has never been thoroughly explored. We report the successful fabrication of microelectrode biosensors based on glucose oxidase, d-amino acid oxidase, and glutamate oxidase immobilized using PEGDE. We found that biosensors made with PEGDE exhibited high sensitivity and a response time on the order of seconds, which is sufficient for observing biological processes in vivo. The enzymatic activity on these biosensors was highly stable over several months when they were stored at 4 °C, and over at least 3d at 37 °C. Glucose microelectrode biosensors implanted in the central nervous system of anesthetized rats reliably monitored changes in brain glucose levels induced by sequential administration of insulin and glucose. PEGDE provides a simple, low cost, non-toxic alternative for the preparation of in vivo microelectrode biosensors.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP72207808	Poly(ethylene glycol) diglycidyl ether		72207-80-8	Price

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