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Cross-peptide Bond 13C--15N Coupling Constants by 13C and J Cross-Polarization 15N NMR

Cross-peptide Bond 13C--15N Coupling Constants by 13C and J Cross-Polarization 15N NMR

C Niu, R D Bertrand, H Shindo, J S Cohen

J Biochem Biophys Methods. 1979 Jul;1(3):135-43.

PMID: 552384

Abstract:

Comparative 13C--15N coupling constants are reported for the linear dipeptide tBoc-L-[U-13C]Ala-[15N]GlyOMe and the corresponding cyclic diketopiperazine, both in dimethylsulfoxide (DMSO) and, upon removal of the tBoc group, in water solutions. Spectra were obtained by 13C NMR and by the first application of J cross-polarization (JCP) 15N NMR, which greatly reduces the time required to accumulate 15N NMR spectra. In DMSO there was evidence for the formation of complexed species which were not present in water. The values obtained for the cross-peptide bond coupling constant 2J13C alpha--15N were consistently less (by 2.2 Hz in DMSO, 4.3 Hz in water) for the cyclic than for the linear peptide, which may be related to the cross-peptide bond conformation. The 15N resonance for the cyclic peptide was shifted only 2 ppm downfield from the linear peptide chemical shift value in both solvents.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP285978247	Glyphosate-2-13C,15N		285978-24-7	Price

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