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Cross-reactivity of Monoclonal Antibodies Against Lipopolysaccharides of Gram-Negative Bacteria

Cross-reactivity of Monoclonal Antibodies Against Lipopolysaccharides of Gram-Negative Bacteria

J de Jongh-Leuvenink, A S Bouter, J H Marcelis, J Schellekens, J Verhoef

Eur J Clin Microbiol. 1986 Apr;5(2):148-51.

PMID: 2424754

Abstract:

Monoclonal antibodies were produced against Escherichia coli O111, Escherichia coli J5, and the rough (R) mutant of Salmonella typhimurium M206, and tested by enzyme-linked immunosorbent assay against lipopolysaccharides of several gram-negative strains. The monoclonal antibodies were also identified with an immunoblotting assay. Anti-Escherichia coli O111 monoclonal antibodies reacted only with homologous O antigens. Anti-J5 monoclonal antibodies cross-reacted with core lipopolysaccharide, especially with Rc lipopolysaccharide. IgM anti-J5 monoclonal antibodies showed more extensive cross-reactivity than IgG3 monoclonal antibodies. Anti-Re monoclonal antibodies cross-reacted weakly with all rough lipopolysaccharide tested. Thus, the varying specificity of these monoclonal antibodies seems to indicate that the core regions in the lipopolysaccharides of various gram-negative bacteria are not similar.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
LS7413110	Lipopolysaccharides (rough strains) from Escherichia coli J5 (Rc mutant)			Price

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