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Cross-reactivity of the anti-PML Antibody PG-M3 With the Herpes Simplex Virus Type 1 Immediate Early Protein ICP4

Cross-reactivity of the anti-PML Antibody PG-M3 With the Herpes Simplex Virus Type 1 Immediate Early Protein ICP4

S L Boulware, P C Weber

J Gen Virol. 2000 Jul;81(Pt 7):1773-7.

PMID: 10859383

Abstract:

The PML protein is one of the components of ND10, nuclear matrix-associated structures which undergo rapid disintegration at the onset of herpes simplex virus type 1 (HSV-1) infection. This disruption event has been frequently visualized in immunofluorescence assays using the anti-PML mouse monoclonal antibody PG-M3. This antibody was surprisingly found to also stain nuclear virus replication compartments when employed at higher concentrations. This was shown to be due to an unexpected cross-reactivity of the PG-M3 antibody with the HSV-1 immediate early protein ICP4, a known component of replication compartments. The sequences of ICP4 recognized by PG-M3 were found to map to the extreme amino-terminal end of the protein, which includes a 21 amino acid segment that is partially homologous to the peptide of PML that was used to make PG-M3. These results suggest that PG-M3 may no longer represent an appropriate antibody for use in visualizing the fate of PML and ND10 during HSV-1 infection.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
IAR4246221	Anti-PML antibody, Mouse monoclonal			Price

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