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Crystallization and Preliminary Crystallographic Analysis of the Catalytic Domain Cellobiohydrolase I From Talaromyces Emersonii

Alice Grassick, Gabriel Birrane, Maria Tuohy, Patrick Murray, Timothy Higgins

Acta Crystallogr D Biol Crystallogr. 2003 Jul;59(Pt 7):1283-4.

PMID: 12832787

Abstract:

Cellobiohydrolase IB is the first native enzyme from the filamentous fungus Talaromyces emersonii to be crystallized. It is a highly thermostable exo-acting enzyme. The native enzyme (MW = 56 kDa) was crystallized using the hanging-drop vapour-diffusion method with ammonium phosphate (dibasic) as a precipitant at pH 8.5. The crystal belongs to the tetragonal space group P4(1)2(1)2, with unit-cell parameters a = b = 74.43, c = 176.92 A, and diffracted to 1.77 A resolution at room temperature.

Chemicals Related in the Paper:

Catalog Number Product Name Structure CAS Number Price
AP7783280-A Ammonium phosphate dibasic Ammonium phosphate dibasic 7783-28-0 Price
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