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Crystallization and Preliminary X-ray Crystallographic Study on a Xyloglucan-Specific Exo-Beta-Glycosidase, Oligoxyloglucan Reducing-End Specific Cellobiohydrolase

Katsuro Yaoi, Hidemasa Kondo, Mamoru Suzuki, Natsuko Noro, Sakae Tsuda, Yasushi Mitsuishi

Acta Crystallogr D Biol Crystallogr. 2003 Oct;59(Pt 10):1838-9.

PMID: 14501131

Abstract:

A novel xyloglucan-specific exo-beta-glycosidase, oligoxyloglucan reducing-end specific cellobiohydrolase (OXG-RCBH), recognizes the reducing end of oligoxyloglucan and releases two glucosyl residue segments from the main chain. OXG-RCBH was crystallized by the hanging-drop vapour-diffusion method with polyethylene glycol 3000 and polyethylene glycol 400 as precipitants. The crystals belong to the orthorhombic space group P2(1)2(1)2(1), with unit-cell parameters a = 61.0, b = 146.9, c = 211.9 A. The crystals diffract to a resolution of 2.2 A and are suitable for X-ray structure analysis.

Chemicals Related in the Paper:

Catalog Number Product Name Structure CAS Number Price
AP25322683-E Polyethylene glycol 3000 Polyethylene glycol 3000 25322-68-3 Price
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