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Culture of Escherichia Coli in SOC Medium Improves the Cloning Efficiency of Toxic Protein Genes

Culture of Escherichia Coli in SOC Medium Improves the Cloning Efficiency of Toxic Protein Genes

Qiao-Yang Sun, Ling-Wen Ding, Liang-Liang He, Yong-Bin Sun, Jun-Li Shao, Ming Luo, Zeng-Fu Xu

Anal Biochem. 2009 Nov 1;394(1):144-6.

PMID: 19622338

Abstract:

In this paper, we report a useful protocol for cloning toxic protein genes. Use of the SOC medium, which is a glucose-containing rich medium, significantly improved the transformation efficiency of a recombinant plasmid containing a toxic plant subtilase SaSBT1 cDNA. Both glucose and rich nutrients present in the SOC medium prevented the unintended activation of the lac promoter carried on the cloning vector, and led to significantly improved transformation efficiency of recombinant plasmids containing toxic protein genes and an increased rate of transformant growth.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
IAR42411427	SOC Medium			Price

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