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Cultured Human and Rat Oligodendrocytes and Rat Schwann Cells Do Not Have Immune Response Gene Associated Antigen (Ia) on Their Surface

R P Lisak, M Hirayama, D Kuchmy, A Rosenzweig, S U Kim, D E Pleasure, D H Silberberg

Brain Res. 1983 Dec 19;289(1-2):285-92.

PMID: 6362776

Abstract:

In order to determine if oligodendrocytes or Schwann cells had surface immune response gene associated antigen (Ia), we studied the binding of: (a) mouse monoclonal antibodies to rat Ia, to cultures of rat oligodendrocytes and Schwann cells; and, (b) mouse monoclonal antibodies to human Ia, to cultures of human oligodendrocytes employing radioimmunoassay and indirect immunofluorescence. Cells were identified using phenotypic markers; rabbit anti-galactocerebroside (GalC) for oligodendrocytes; rabbit anti-GalC and rabbit anti-Schwann cell for Schwann cells; rabbit anti-glial fibrillary acidic protein for astrocytes; rabbit anti-fibronectin for fibro-blasts and leptomeningeal cells, and the capacity to ingest latex particles for macrophage-microglia. Ia could not be detected on the surface of oligodendrocytes, Schwann cells, astrocytes, fibroblasts, or leptomeningeal cells. A small number of latex ingesting cells bound anti-Ia even after blocking of surface Fc receptors.

Chemicals Related in the Paper:

Catalog Number Product Name Structure CAS Number Price
IAR42414302 Anti-Glial Fibrillary Acidic Protein antibody, Mouse monoclonal Anti-Glial Fibrillary Acidic Protein antibody, Mouse monoclonal Price
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