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Cutting Edge: Quantitative Determination of CD40L Threshold for IL-12 and IL-23 Production From Dendritic Cells

Cutting Edge: Quantitative Determination of CD40L Threshold for IL-12 and IL-23 Production From Dendritic Cells

Kaveh Abdi, Karen Laky, Kartika Padhan, Constantinos Petrovas, Jeff Skinner, Juraj Kabat, David W Dorward, Joseph Brzostowski, Eric O Long, Giorgio Trinchieri, Rajat Varma

J Immunol. 2018 Nov 15;201(10):2879-2884.

PMID: 30315139

Abstract:

Early secretion of IL-12 by mouse dendritic cells (DCs) instructs T cells to make IFN-γ. However, only activated, but not naive T cells are able to license DCs for IL-12 production. We hypothesized that it might be due to different levels of CD40L expression on the surface of these cells, as CD40 signals are required for IL-12 production. Using quantitative cell-free systems incorporating CD40L in lipid bilayers combined with total internal reflection fluorescence microscopy and flow cytometry, we show that as low as ∼200 CD40L molecules/μm2 in combination with IL-4 is sufficient to induce IL-12 production by DCs. Remarkably, CD40L alone is adequate to induce IL-23 secretion by DCs. Thus, although activated T cells have somewhat higher levels of CD40L, it is the combination of CD40L and the cytokines they secrete that licenses DCs and influences the effector class of the immune response.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
IAR4248660	IL-12 from mouse			Price

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