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Cy5.5-Conjugated Anti-Human CD31 Monoclonal Antibody

Cy5.5-Conjugated Anti-Human CD31 Monoclonal Antibody

Arvind Chopra

PMID: 20641501

Abstract:

The CD31 molecule, also known as the platelet endothelial cell (EC) adhesion molecule 1, is a 130-kD transmembrane glycoprotein that is a member of the immunoglobulin superfamily. This glycoprotein is expressed primarily on the EC intercellular junctions in the vascular system, but it is also expressed to a varying extent on other cell types (1, 2). The ECs are believed to play a major role in a variety of physiological processes such as coagulation, vascular tone, inflammation, and angiogenesis (1). Although CD31 is expressed in several leukocyte cell subtypes and in platelets, it is considered to be a reliable marker for tumor vasculature (3, 4). Runnels et al. showed that fluorescence-labeled rat anti-mouse CD31 monoclonal antibodies (MAbs) targeting the CD31 molecule could be used for the in vivo imaging of EC dynamics in mice (5). The investigators showed that the MAbs could stain the ECs in the vascular wall for a period of 24 to 48 h after administration. On the basis of these observations, Bogdanov et al. designed a similar study using the anti-human CD31 MAb labeled with the near-infrared fluorescent dye Cy5.5 (Cy5.5 anti-hCD31) to investigate the detection of human EC-lined blood vessels developed in an animal model using human umbilical vein EC (HUVEC) Matrigel implants (6). To perform this study the investigators assumed that the human anti-CD31 MAb did not cross-react with the mouse ECs and had a high affinity for the targeted marker protein, that the CD31 was constitutively expressed on the surface of ECs, and that the MAb was internalized at a slow rate.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
IAR42414161	Anti-CD31(PECAM-1) antibody , Mouse monoclonal			Price

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