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Cyanin Chloride Inhibits Hyperbaric Pressure-Induced Decrease of Intracellular Glutamate-Aspartate Transporter in Rat Retinal Müller Cells

Cyanin Chloride Inhibits Hyperbaric Pressure-Induced Decrease of Intracellular Glutamate-Aspartate Transporter in Rat Retinal Müller Cells

Xiaomin Chen, Yue Wang, Fangfang Han, Min Ke

J Ophthalmol. 2018 Oct 31;2018:6128470.

PMID: 30515320

Abstract:

Purpose:
Glaucoma is the leading cause of irreversible blindness throughout the world. The pathogenesis of glaucoma is complex, and neuroprotection is a crucial aspect of therapy. High concentrations of extracellular glutamate are toxic to the optic nerve. The glutamate-aspartate transporter (GLAST) in retinal Müller cells is involved in the development of glaucoma. Anthocyanin has been reported to protect retinal neurons. We hypothesize that cyanin chloride, a type of anthocyanin, can inhibit hyperbaric pressure-induced GLAST decreases in cultured rat retinal Müller cells and may serve as a potential neuroprotective agent in glaucoma treatment.
Materials and methods:
Sprague Dawley rat Müller cells were cultured in a hyperbaric pressure device at 60 mmHg additional pressure and treated with cyanin chloride (10 μmol/L, 30 μmol/L, or 50 μmol/L) or vehicle for 2 hours. Cell survival rates (SRs) were evaluated by an MTT assay. GLAST mRNA and protein expression were determined by western blot and RT-PCR analyses, respectively.
Results:
Cell SR was significantly decreased in the 60 mmHg additional hyperbaric pressure group compared to the control group (P < 0.01). Cyanin chloride treatment significantly improved SR under 60 mmHg additional pressure (P < 0.01). GLAST mRNA and protein expression levels in Müller cells were significantly reduced in the 60 mmHg hyperbaric pressure group compared to the control group (P < 0.01), but cyanin chloride significantly inhibited hyperbaric pressure-induced decreases in GLAST expression (P < 0.01).
Conclusion:
Our results support our hypothesis and demonstrate that cyanin chloride can protect rat retinal Müller cells from hyperbaric pressure-induced decreases of GLAST.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP2611678-A	Cyanin chloride		2611-67-8	Price

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