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Cytotoxicity and Apoptotic Effects of Polyphenols From Sugar Beet Molasses on Colon Carcinoma Cells in Vitro

Cytotoxicity and Apoptotic Effects of Polyphenols From Sugar Beet Molasses on Colon Carcinoma Cells in Vitro

Mingshun Chen, Zhengang Zhao, Shujuan Yu

Int J Mol Sci. 2016 Jun 23;17(7):993.

PMID: 27347927

Abstract:

Three polyphenols were isolated and purified from sugar beet molasses by ultrasonic-aid extraction and various chromatographic techniques, and their structures were elucidated by spectral analysis. Cytotoxicity and the molecular mechanism were measured by methyl thiazolyl tetrazolium (MTT) assay, flow cytometry, caspase-3 activity assay and Western blot assay. The results showed that gallic acid, cyanidin-3-O-glucoside chloride and epicatechin have cytotoxicity to the human colon, hepatocellular and breast cancer cells. Cyanidin-3-O-glucoside chloride showed its cytotoxicity against various tumor cell lines, particularly against colon cancer Caco-2 cells with half maximal inhibitory concentration (IC50) value of 23.21 ± 0.14 μg/mL in vitro. Cyanidin-3-O-glucoside chloride may be a potential candidate for the treatment of colon cancer. In the mechanism study, cyanidin-3-O-glucoside chloride increased the ratio of cell cycle at G₀/G₁ phase and reduced cyclin D1 expression on Caco-2 cells. Cyanidin-3-O-glucoside chloride decreased mutant p21 expression, and increased the ratio of Bax/Bcl-2 and the activation of caspase-3 to induce apoptosis.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP7084244-B	Cyanidin 3-O-glucoside chloride		7084-24-4	Price

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