Home
Resources
Publications
d-1,2,4-Butanetriol Production From Renewable Biomass With Optimization of Synthetic Pathway in Engineered Escherichia Coli

d-1,2,4-Butanetriol Production From Renewable Biomass With Optimization of Synthetic Pathway in Engineered Escherichia Coli

Xin Wang, Nana Xu, Shewei Hu, Jianming Yang, Qian Gao, Sheng Xu, Kequan Chen, Pingkai Ouyang

Bioresour Technol. 2018 Feb;250:406-412.

PMID: 29195152

Abstract:

Bio-based production of d-1,2,4-butanetriol (BT) from renewable substrates is increasingly attracting attention. Here, the BT biosynthetic pathway was constructed and optimized in Escherichia coli to produce BT from pure d-xylose or corncob hydrolysates. First, E. coli BL21(DE3) was identified as a more proper host for BT production through host screening. Then, BT pathway was systematically optimized with gene homolog screening strategy, mainly targeting three key steps from xylonic acid to BT catalyzed by d-xylonate dehydratase (XD), 2-keto acid decarboxylase (KDC) and aldehyde reductase (ALR). After screening six ALRs, four KDCs and four XDs, AdhP from E. coli, KdcA from Lactococcus lactis and XylD from Caulobacter crescentus were identified more efficiently for BT production. The co-expression of these enzymes in recombinant strain BL21-14 led to BT production of 5.1 g/L under the optimized cultivation conditions. Finally, BT production from corncob hydrolysates was achieved with a titer of 3.4 g/L.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP3068006	(±)-1,2,4-Butanetriol		3068-00-6	Price

As a specialist in analytical chemical Products, Alfa Chemistry offers consumers a wealth of raw materials and a full range of technologies and services.

QUICK LINKS

HEADQUARTERS

Tel:

Fax:

Email: