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Decolorization Potential of Some Reactive Dyes With Crude Laccase and Laccase-Mediated System

Decolorization Potential of Some Reactive Dyes With Crude Laccase and Laccase-Mediated System

Samet Saşmaz, Serap Gedikli, Pınar Aytar, Gökhan Güngörmedi, Ahmet Cabuk, Evrim Hür, Arzu Unal, Nazif Kolankaya

Appl Biochem Biotechnol. 2011 Feb;163(3):346-61.

PMID: 20669054

Abstract:

In this study, decolorization of dyestuffs, such as Reactive Red 198, Rem Blue RR, Dylon Navy 17, Rem Red RR, and Rem Yellow RR was studied using laccase and laccase-mediated system. The laccases are known to have an important potential for remediation of pollutants. Among these dyestuffs, decolorization of Rem Blue RR and Dylon Navy 17 was performed with crude laccase under optimized conditions. Vanillin was selected as laccase mediator after screening six different compounds with Rem Yellow RR, Reactive Red 198, and Rem Red RR as substrates. However, Rem Yellow RR was not decolorized by either laccase or laccase-mediated system. It is observed that the culture supernatant contained high laccase activity after treatment with catalase that was responsible for the decolorization. Besides, culture supernatant with high laccase activity as enzyme source was treated with catalase; in this way, the hypothesis that laccase was the enzyme responsible for decolorization was supported. The Rem Blue RR was decolorized with 64.84% under the optimum conditions and Dylon Navy 17 with 75.43% with crude laccase. However, using the laccase and vanillin, the decolorization of Reactive Red 198 and Rem Red RR was found to be 62% and 68%, respectively. Our study demonstrated that the decolorization abilities of laccase and/or laccase mediator systems were based on the types of mediator, the dye structure, and the standard experimental conditions. Also, the electrochemical behaviors of some samples were studied. The redox potentials of these samples were determined using cyclic voltammetry on glassy carbon electrode in phosphate buffer (pH 6) solution.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AS26617	Polynuclear aromatic hydrocarbons 6 solution			Price

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