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Decorrelation Correction for Nanoparticle Tracking Analysis of Dilute Polydisperse Suspensions in Bulk Flow

Decorrelation Correction for Nanoparticle Tracking Analysis of Dilute Polydisperse Suspensions in Bulk Flow

John Hartman, Brian Kirby

Phys Rev E. 2017 Mar;95(3-1):033305.

PMID: 28415349

Abstract:

Nanoparticle tracking analysis, a multiprobe single particle tracking technique, is a widely used method to quickly determine the concentration and size distribution of colloidal particle suspensions. Many popular tools remove non-Brownian components of particle motion by subtracting the ensemble-average displacement at each time step, which is termed dedrifting. Though critical for accurate size measurements, dedrifting is shown here to introduce significant biasing error and can fundamentally limit the dynamic range of particle size that can be measured for dilute heterogeneous suspensions such as biological extracellular vesicles. We report a more accurate estimate of particle mean-square displacement, which we call decorrelation analysis, that accounts for correlations between individual and ensemble particle motion, which are spuriously introduced by dedrifting. Particle tracking simulation and experimental results show that this approach more accurately determines particle diameters for low-concentration polydisperse suspensions when compared with standard dedrifting techniques.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AS211448	Polydisperse Particle Standard			Price

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