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Degradation of Acetochlor by Consortium of Two Bacterial Strains and Cloning of a Novel Amidase Gene Involved in Acetochlor-Degrading Pathway

Degradation of Acetochlor by Consortium of Two Bacterial Strains and Cloning of a Novel Amidase Gene Involved in Acetochlor-Degrading Pathway

Yi Li, Qing Chen, Cheng-Hong Wang, Shu Cai, Jian He, Xing Huang, Shun-Peng Li

Bioresour Technol. 2013 Nov;148:628-31.

PMID: 24075675

Abstract:

Two bacterial strains Sphingobium quisquiliarum DC-2 and Sphingobium baderi DE-13 were isolated from activated sludge. Acetochlor was transformed by S. quisquiliarum DC-2 to a transitory intermediate 2-chloro-N-(2-methyl-6-ethylphenyl)acetamide (CMEPA), which was further transformed to 2-methyl-6-ethylaniline (MEA), and MEA could not be degraded by strain DC-2. S. baderi DE-13, incapable of degrading acetochlor, showed capability of degrading MEA to an intermediate 2-methyl-6-ethylaminophenol (MEAOH). MEAOH was further transformed to 2-methyl-6-ethylbenzoquinoneimine (MEBQI), which was mineralized by strain DE-13. A gene, cmeH, encoding an amidase that catalyzed the amide bond cleavage of CMEPA was cloned from strain DC-2. CmeH was expressed in Escherichia coli BL21 and homogenously purified using Ni-nitrilotriacetic acid affinity. CmeH efficiently hydrolyzed CMEPA and other important herbicide, such as propanil, fenoxaprop-p-ethyl and clodinafop-propargyl.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP1189897446	Acetochlor-(2-ethyl-6-methylphenyl-d11)		1189897-44-6	Price

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