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Dengue Virus Photo-Inactivated in Presence of 1,5-iodonaphthylazide (INA) or AMT, a Psoralen Compound (4'-aminomethyl-trioxsalen) Is Highly Immunogenic in Mice

Kanakatte Raviprakash, Peifang Sun, Yossef Raviv, Thomas Luke, Nicholas Martin, Tadeusz Kochel

Hum Vaccin Immunother. 2013 Nov;9(11):2336-41.

PMID: 23835446

Abstract:

Two novel methods of dengue virus inactivation using iodonaphthyl azide (INA) and aminomethyl trioxsalen (AMT) were compared with traditional virus inactivation by formaldehyde. The AMT inactivated dengue-2 virus retained its binding to a panel of 5 monoclonal antibodies specific for dengue-2 envelope protein, whereas inactivation by formaldehyde and INA led to 30-50% decrease in binding. All three inactivated viruses elicited high level virus neutralizing antibodies in vaccinated mice. However, only mice vaccinated with AMT inactivated virus mounted T cell responses similar to live, uninactivated virus.

Chemicals Related in the Paper:

Catalog Number Product Name Structure CAS Number Price
AP3902714-A Trioxsalen Trioxsalen 3902-71-4 Price
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