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Derivatization of Isolated Endogenous Butyrobetaine With 4'-bromophenacyl Trifluoromethanesulfonate Followed by High-Performance Liquid Chromatography

S Krahenbuhl, P E Minkler, C L Hoppel

J Chromatogr. 1992 Jan 3;573(1):3-10.

PMID: 1564104

Abstract:

A method for the isolation and chromatography of butyrobetaine from plasma, urine, and liver is described. The recovery of [3H-methyl]butyrobetaine from spiked biological samples was from 76-80%. Spiked samples then were derivatized with 4'-bromophenacyl trifluoromethanesulfonate and the butyrobetaine 4'-bromophenacyl ester was isolated by high-performance liquid chromatography (HPLC). Radioactivity eluted in a single peak which co-chromatographed with authentic butyrobetaine 4'-bromophenacyl ester. Two identical liver specimens were treated according to this isolation procedure. Prior to derivatization, one specimen was treated with butyrobetaine hydroxylase. After derivatization, there was no butyrobetaine 4'-bromophenacyl ester peak in the specimen treated with butyrobetaine hydroxylase. The HPLC detection sensitivity to butyrobetaine 4'-bromophenacyl ester was 1 pmol injected with a signal-to-noise greater than 2:1.

Chemicals Related in the Paper:

Catalog Number Product Name Structure CAS Number Price
AP93128042 4-Bromophenacyl trifluoromethanesulfonate 4-Bromophenacyl trifluoromethanesulfonate 93128-04-2 Price
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