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Derivatization Strategies for the Determination of Biogenic Amines in Wines by Chromatographic and Electrophoretic Techniques

Derivatization Strategies for the Determination of Biogenic Amines in Wines by Chromatographic and Electrophoretic Techniques

Santiago Hernández-Cassou, Javier Saurina

J Chromatogr B Analyt Technol Biomed Life Sci. 2011 May 15;879(17-18):1270-81.

PMID: 21185242

Abstract:

This paper revises the derivatization approaches for the determination of biogenic amines in wines. Since most of these amines display poor spectroscopic features to be detected by UV absorption or emission (fluorescence) spectroscopy, derivatization is necessary to attain the desired sensitivity. Reagents such as o-phthaldialdehyde, fluorenylmethylchloroformate, dansyl-Cl and dabsyl-Cl have widely been used for analytical labeling through amino group. A comparison of features of off- and on-line pre- and post chromatographic/electrophoretic labeling is given using 1,2-naphthoquinone-4-sulfonate (NQS) as an example. The evaluation of the influence of the wine sample composition on the derivatization process indicates that pre-column labeling may undergo more severe matrix effects.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP643798	Phthaldialdehyde		643-79-8	Price

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