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Dermatan Sulfate Oligosaccharides Having Reducing End 2, 5-anhydro-d-talose Inhibit Bovine Testicular Hyaluronidase Activity

Dermatan Sulfate Oligosaccharides Having Reducing End 2, 5-anhydro-d-talose Inhibit Bovine Testicular Hyaluronidase Activity

Ikuko Kakizaki, Hideyo Koizumi, Takashi Kobayashi, Toshiya Nakamura, Mitsuo Majima

Carbohydr Res. 2019 Sep 1;483:107754.

PMID: 31398613

Abstract:

Dermatan sulfate oligosaccharides having reducing end 2,5-anhydro-d-talose were prepared by partial N-deacetylation of dermatan sulfate polysaccharide with hydrazine followed by deamination with nitrous acid, and the effect of these oligosaccharides on the activity of bovine testicular hyaluronidase was investigated. Hydrolysis activity and transglycosylation activity of this enzyme were assessed in an independent reaction system by analyzing the products by HPLC. Dermatan sulfate oligosaccharides inhibited hyaluronan hydrolysis by bovine testicular hyaluronidase. Kinetic analysis of the hydrolysis reaction revealed that the inhibition mode by dermatan sulfate oligosaccharides was as competitive as that previously shown by chondroitin sulfate oligosaccharides. Transglycosylation of hyaluronan by bovine testicular hyaluronidase, as a reverse reaction of hydrolysis of hyaluronan, was also inhibited. These inhibitory effects were dependent on the dose and sulfation degree of dermatan sulfate.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP54328335-A	Dermatan sulfate		54328-33-5	Price

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