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Design and Synthesis of a Tat-related Gene Transporter: A Tool for Carrying the Adenovirus Vector Into Cells

Design and Synthesis of a Tat-related Gene Transporter: A Tool for Carrying the Adenovirus Vector Into Cells

Shinya Kida, Mitsuko Maeda, Keiko Hojo, Yusuke Eto, Jian-Qing Gao, Shinnosuke Kurachi, Hiroyuki Mizuguchi, Takao Hayakawa, Tadanori Mayumi, Shinsaku Nakagawa, Koichi Kawasaki

Bioorg Med Chem Lett. 2006 Feb;16(3):743-5.

PMID: 16337377

Abstract:

A Tat-related peptide, acetyl-Gly-Arg-Arg-Arg-Arg-Arg-Gln-Arg-Arg-Arg-Pro-Pro-Gln-Gly-Cys amide, designed to transport an Adenovirus vector (Ad) into cells, was synthesized. The synthetic peptide was conjugated to Ad, which potentially can act as an efficient carrier of heterologous genes into cells. The Tat-related peptide was synthesized using the solid phase method and then was coupled to the heterofunctional cross-linking reagent, 6-maleimidohexanoic acid N-hydroxysuccinimide ester. The resulting peptide-succinimidohexanoic acid N-hydroxysuccinimide ester was conjugated to Ad containing the luciferase gene. B16BL6 cells infected with the peptide-conjugated Ad luciferase gene construct exhibit a 50-fold greater luciferase activity than B16BL6 cells infected with wild-type Ad containing the luciferase gene.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP55750635	6-Maleimidohexanoic acid N-hydroxysuccinimide ester		55750-63-5	Price

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