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Destaining of Coomassie Brilliant Blue R-250-stained Polyacrylamide Gels With Fungal Laccase

Destaining of Coomassie Brilliant Blue R-250-stained Polyacrylamide Gels With Fungal Laccase

Jie Yang, Xiaodan Yang, Xiuyun Ye, Juan Lin

Anal Biochem. 2016 Jan 15;493:27-9.

PMID: 26475566

Abstract:

An enzyme-based method for destaining polyacrylamide gels stained with Coomassie Brilliant Blue R-250 is described. Distilled water supplemented with diluted fermentation broth of a laccase-producing white-rot fungus, Cerrena sp., was used for gel destaining, and a clear gel background was obtained in 2 h at 37 °C. Sensitivity of protein detection was 10 ng. The method did not require organic solvents or changing the destaining solution. Due to simultaneous gel destaining and dye decolorization, the colorless destaining solution can be disposed of directly. Laccase destaining of polyacrylamide gels was simple, efficient, and environmentally friendly.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP37340548	Hide-Remazol Brilliant Blue R		37340-54-8	Price
AP6104592	Brilliant Blue R		6104-59-2	Price

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