Home
Resources
Publications
Detection Limits of Electron and Electron Capture Negative Ionization-Mass Spectrometry for Aldehydes Derivatized With o-(2,3,4,5,6-pentafluorobenzyl)-hydroxylamine Hydrochloride

Detection Limits of Electron and Electron Capture Negative Ionization-Mass Spectrometry for Aldehydes Derivatized With o-(2,3,4,5,6-pentafluorobenzyl)-hydroxylamine Hydrochloride

Josef Beránek, Darrin A Muggli, Alena Kubátová

J Am Soc Mass Spectrom. 2010 Apr;21(4):592-602.

PMID: 20106680

Abstract:

In contrast to common expectations, the differences in limits of detection (LODs) between electron capture negative ionization (ECNI) and electron ionization (EI) mass spectrometry (MS) were found to be insignificant for a wide range of aldehydes derivatized with o-(2,3,4,5,6-pentafluorobenzyl)-hydroxylamine hydrochloride. Comparison of the two ionization methods based on LOD confidence intervals revealed that a traditional presentation of the LOD or limit of quantitation (LOQ) as a single value may over/underestimate the significance of obtained results. LODs were between 20 and 150 pg injected for the majority of tested derivatized carbonyls using both ionization methods. ECNI-MS improved LODs by approximately 10- to 20-fold only for two derivatized aldehydes, 4-hydroxybenzaldehyde and 5-(hydroxymethyl)furfural. Selectivity of ECNI did not appear to be beneficial when analyzing a wood smoke particulate matter (WS-PM) extract, possibly because the majority of interferences were removed during sample preparation (i.e., liquid-liquid extraction). The impact of four different data acquisition modes of transmission quadrupole (TQ)-MS on LODs and their precisions was also investigated. As expected, LODs in the selected ion monitoring (SIM) were approximately two to four times lower than those obtained using total ion current (TIC) mode. More importantly, TQ-MS in the selected ion-total ion (SITI) mode (i.e., acquiring SIM and TIC data in a single analysis) provided signal-to-noise ratios and precisions, which were comparable to SIM alone.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP57981029	O-(2,3,4,5,6-Pentafluorobenzyl)hydroxylamine hydrochloride		57981-02-9	Price

As a specialist in analytical chemical Products, Alfa Chemistry offers consumers a wealth of raw materials and a full range of technologies and services.

QUICK LINKS

HEADQUARTERS

Tel:

Fax:

Email: