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Detection of Nepsilon-monomethyllysine Using High-Performance Liquid Chromatography and High-Performance Liquid Chromatography-Mass Spectrometry

Detection of Nepsilon-monomethyllysine Using High-Performance Liquid Chromatography and High-Performance Liquid Chromatography-Mass Spectrometry

H Kalász, Z Szücs, M Tihanyi, A Szilágyi, J Lengyel

J Chromatogr A. 2005 Jun 24;1079(1-2):208-12.

PMID: 16038306

Abstract:

Nepsilon-Monomethyllysine was identified in the serum, urine, brain, and liver samples of rats treated per os with L-deprenyl. The identification procedure included reaction with Fmoc chloride, clean-up, and analysis using HPLC-UV-MS. Oral administration of (-)-N-14C-methyl-N-propynyl(2-phenyl-1-methyl)ethylammonium hydrochloride L-deprenyl) to rats resulted in transfer of the radiolabelled methyl group to the Nepsilon-amino group of the endogenous lysine. The radiolabelled Nepsilon-monomethyllysine was urinary eliminated together with the other radiolabelled deprenyl metabolites, such as deprenyl-N-oxide and methamphetamine. The presence of Nepsilon-monomethyllysine has also been traced, and its concentrations were compared in the serum, liver and brain of rats subjected to L-deprenyl treatment. Methyl group transfer from the L-deprenyl to endogenous compounds; and the urinary elimination of their products may offer a vital way to eliminate or to decrease the degree of drug transmethylation to the lysine constituents of blood vessels' proteins.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP28920436	Fmoc chloride		28920-43-6	Price

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