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Determination and Characterization of Succinyl Tri-Alanine P-Nitroanilide Hydrolyzing Metalloendopeptidase in Serum

M Ishida, M Ogawa, T Mori, T Mega

Enzyme. 1987;37(4):202-7.

PMID: 3111843

Abstract:

Serum succinyl (Ala)3-p-nitroanilide hydrolyzing elastase-like activity which elevates in patients with obstructive jaundice, is due to the joint action of two enzymes: first, succinyl (Ala)3-p-nitroanilide is cleaved to succinyl (Ala)2 and Ala-p-nitroanilide by metalloendopeptidase, and then Ala-p-nitroanilide is cleaved to Ala and p-nitroaniline by aminopeptidase. We adopt a new assay method for serum endopeptidase activity using HPLC.

Chemicals Related in the Paper:

Catalog Number Product Name Structure CAS Number Price
AP108322038 N-Succinyl-Ala-Ala-Val-Ala p-nitroanilide N-Succinyl-Ala-Ala-Val-Ala p-nitroanilide 108322-03-8 Price
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