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Determination of 2-hydroxypropyl-gamma-cyclodextrin in Plasma of Cynomolgus Monkeys After Oral Administration by Gas Chromatography-Mass Spectrometry

Determination of 2-hydroxypropyl-gamma-cyclodextrin in Plasma of Cynomolgus Monkeys After Oral Administration by Gas Chromatography-Mass Spectrometry

W Blum, R Aichholz, P Ramstein, A Fetz, F Raschdorf

J Chromatogr B Biomed Sci Appl. 1998 Dec 11;720(1-2):171-8.

PMID: 9892079

Abstract:

This report describes a specific and highly sensitive gas chromatography-mass spectrometry (GC-MS) assay for the analysis of industrially produced 2-hydroxypropyl-gamma-cyclodextrin, a heterogeneous mixture of homologues and isomers, in plasma of cynomolgus monkeys. Instead of analyzing the polysaccharide mixture as a whole, in a first step the HP-gamma-cyclodextrin mixture, together with the internal standard (2,6-di-O-methyl-beta-cyclodextrin), was deuteromethylated, and in a second step hydrolyzed with hydrochloric acid to the respective monosaccharides. The resulting reaction mixture was trimethylsilylated to 1,4-bis(O-trimethylsilyl)-2,3-bis-O-deuteromethyl-6-O-2'-deuter omethoxypropylglucose (representative for HP-gamma-CD) and 1,4-bis-(O-trimethylsilyl)-bis-2,6-O-methyl-3-O-deuteromethylglucose+ ++ (representative for the internal standard), respectively, and analyzed by GC-MS. The limit of quantification of this assay was 20 nmol/l.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP128446344	(2-Hydroxypropyl)-γ-cyclodextrin		128446-34-4	Price

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