Home
Resources
Publications
Determination of 4(5)-methylimidazole in Carbonated Beverages by Isotope-Dilution Liquid Chromatography-Tandem Mass Spectrometry

Determination of 4(5)-methylimidazole in Carbonated Beverages by Isotope-Dilution Liquid Chromatography-Tandem Mass Spectrometry

Geemitha Ratnayake, Thor Halldorson, Lianna Bestvater, Gregg T Tomy

Food Addit Contam Part A Chem Anal Control Expo Risk Assess. 2015;32(7):1075-82.

PMID: 25994392

Abstract:

The purpose of this study was to develop a method to quantify 4(5)-methylimidazole (4-MEI), a suspected carcinogen, in carbonated beverages by simple sample dilution and isotope-dilution reverse-phase LC-MS/MS. Isotope dilution using hexa-deuterated methylimidazole (d6-4-MEI) was used to quantify native 4-MEI and to assess matrix effects quantitatively. The accuracy of the method was assessed by intentionally fortifying a negative control sample at three doses: low, medium and high (replicates of n = 5 each) with a known amount of 4-MEI. The respective absolute error in each case was 18.7 ± 0.7%, 14.6 ± 2.8% and 21.1 ± 9.7%. Within-day (intra-) and day-to-day (inter-) repeatability, determined as the relative standard deviation by fortifying a negative control sample (n = 5), were 9.5% and 15.4%, respectively. Average ion suppression of d6-4-MEI in beer was 63.9 ± 3.2%, while no suppression or enhancement was seen in non-alcoholic samples. The instrument and method limit of detection were calculated as 0.6 and 5.8 ng ml(-1), respectively. 4(5)-Methylimidazole was quantified in a variety of store-bought consumer beverages and it was found that in many of the samples tested consuming a single can of beer would result in intake levels of 4-MEI that exceed the no significant risk guideline of 29 µg day(-1). Conversely, 4-MEI in the samples was orders of magnitude smaller than the European Food Safety Authority acceptable daily intake threshold value of 100 mg kg(-1) bw day(-1).

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP822366	4(5)-Methylimidazole		822-36-6	Price

As a specialist in analytical chemical Products, Alfa Chemistry offers consumers a wealth of raw materials and a full range of technologies and services.

QUICK LINKS

HEADQUARTERS

Tel:

Fax:

Email: