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Determination of 7-amino-flunitrazepam (Ro 20-1815) and 7-amino-desmethylflunitrazepam (Ro 5-4650) in Plasma by High-Performance Liquid Chromatography and Fluorescence Detection

Determination of 7-amino-flunitrazepam (Ro 20-1815) and 7-amino-desmethylflunitrazepam (Ro 5-4650) in Plasma by High-Performance Liquid Chromatography and Fluorescence Detection

Y C Sumirtapura, C Aubert, P Coassolo, J P Cano

J Chromatogr. 1982 Oct 8;232(1):111-8.

PMID: 6128350

Abstract:

A high-performance liquid chromatographic method for the simultaneous determination of 7-amino-flunitrazepam (Ro 20-1815) and 7-amino-desmethylflunitrazepam (Ro 5-4650) in plasma is described. After extraction with an organic solvent, the compounds and their internal standard (7-amino-methylclonazepam or Ro 5-3384) are derivatized with fluorescamine and chromatographed on a reversed-phase muBondapak C18 column using pH 8 buffer solution-acetonitrile (3:1) as mobile phase. The detection is performed by a fluorometer at excitation and emission wavelengths of 390 and 470 nm, respectively. The sensitivity limit is about 1 ng/ml of plasma for both 7-amino-flunitrazepam and 7-amino-desmethylflunitrazepam. The method has been applied to the determination of plasma levels of these substances during pharmacokinetic studies of flunitrazepam, desmethylflunitrazepam and 7-amino-flunitrazepam.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP34084509	7-Aminoflunitrazepam solution		34084-50-9	Price

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