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[Determination of γ-aminobutyric Acid in Human Plasma by LC-MS/MS and Its Preliminary Application to a Human Pharmacokinetic Study]

[Determination of γ-aminobutyric Acid in Human Plasma by LC-MS/MS and Its Preliminary Application to a Human Pharmacokinetic Study]

Yao Chen, Xiao-Jian Dai, Jiang-Bo Du, Kan Zhong, Xiao-Yan Chen, Da-Fang Zhong

Yao Xue Xue Bao. 2014 Nov;49(11):1593-9.

PMID: 25757288

Abstract:

A rapid, sensitive and convenient LC-MS/MS method was developed for the determination of γ-aminobutyric acid (GABA) in human plasma. d2-γ-Aminobutyric acid (d2-GABA) was synthesized as internal standard (IS). After extraction from human plasma by protein precipitation with acetonitrile, all analytes were separated on a Luna HILIC column (100 mm x 3.0 mm, 3 μm) using an isocratic mobile phase of water: acetonitrile: formic acid (20 : 80 : 0.12) with a flow rate of 0.5 mL x min(-1). Acquisition of mass spectrometric data was performed in multiple reaction monitoring mode (MRM) in positive electrospray ionization using the transitions of m/z 104 --> 69 for GABA and m/z 106 --> 71 for d2-GABA. The method was linear in the concentration range of 5.00 to 1 000 ng x mL(-1). The intra- and inter-day precisions were within 9.9%, and accuracy ranged from 99.1% to 104%, within the acceptable limit across all concentrations. The method was successfully applied to a pharmacokinetic study of GABA tablets in healthy Chinese volunteers.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP920423	Formic acid-d2		920-42-3	Price

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