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Determination of Anabolic Steroids by Gas chromatography/negative-ion Chemical Ionization Mass Spectrometry and Gas chromatography/negative-ion Chemical Ionization Tandem Mass Spectrometry With Heptafluorobutyric Anhydride Derivatization

Determination of Anabolic Steroids by Gas chromatography/negative-ion Chemical Ionization Mass Spectrometry and Gas chromatography/negative-ion Chemical Ionization Tandem Mass Spectrometry With Heptafluorobutyric Anhydride Derivatization

M H Choi, B C Chung, W Lee, U C Lee, Y Kim

Rapid Commun Mass Spectrom. 1999;13(5):376-80.

PMID: 10209875

Abstract:

A gas chromatography/mass spectrometry (GC/MS) method is described which uses negative ion chemical ionization (NCI) and tandem mass spectrometry (MS/MS) for the determination of eight anabolic steroids in human urine. Eight anabolic steroids were derivatized by heptafluorobutyric anhydride (HFBA), and were determined using GC/NCI-MS and GC/NCI-MS/MS. The linear correlation coefficients for calibration in NCI-MS/MS were in the range 0.9880-0.9988. This method of derivatization with HFBA for use with GC/NCI was useful in determinations of 19-norandrosterone, boldenone, 19-noretiocholanolone, 2-methylandrosterone, nandrolone, 1-methyleneandrosterone, 1-methylandrosterone, 4-dihydroboldenone and mesterolone. The detection limits of this procedure were 5-20 ppb at a signal-to-noise (S/N) ratio of 3.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP336594	Heptafluorobutyric anhydride		336-59-4	Price

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