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Determination of Bradykinin, Kallidin and Met-Lys-bradykinin by High Performance Liquid Chromatography

R Geiger, R Hell, H Fritz

Hoppe Seylers Z Physiol Chem. 1982 May;363(5):527-30.

PMID: 7095757

Abstract:

A high performance liquid chromatography system was developed for the quantitative separation and determination of bradykinin, kallidin and Met-Lys-bradykinin using a reversed-phase column (Nucleosil 5 C8) and an isocratic buffer system. The lower limit of detection is 20 pmol for bradykinin. The intra-variation coefficient of the assay is between 1.8 and 5.7%. The recovery of bradykinin is higher than 96%. Addition of human serum albumin to the assay system in a concentration of 40% lead to a remarkable reduction of the detectable kinin level, which indicates an adsorption of kinins to serum albumin.

Chemicals Related in the Paper:

Catalog Number Product Name Structure CAS Number Price
AP165174607 Met-Lys-[Ser2, Arg3, Pro5, Arg8]-Bradykinin Met-Lys-[Ser2, Arg3, Pro5, Arg8]-Bradykinin 165174-60-7 Price
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