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[Determination of Calceolarioside B in Caulis Akebiae by RP-HPLC]

[Determination of Calceolarioside B in Caulis Akebiae by RP-HPLC]

Hui-Min Gao, Zhi-Min Wang, Li Qu, Xue-Tao Fu, Lin Li

Zhongguo Zhong Yao Za Zhi. 2007 Mar;32(6):476-8.

PMID: 17552147

Abstract:

Objective:
To establish a quantitative method for calceolarioside B in Caulis Akebiae.
Method:
The samples were separated at 35 degrees C on a Phenomenex Luna C18 column eluted with methanol-water-phosphoric acid (35: 65: 0.05) as mobile phase. Flow rate was at 1.0 mL x min(-1) and the detection wavelength was at 330 nm.
Result:
The calibration curve was linear over the range from 0. 04 to 0. 86 g (r = 0. 9998) and the average recovery was 97.2%. 39 Crude drug materials collected from different areas were determined and the contents of calceolarioside B in Caulis Akebiae were fluctuated from 0. 021% to 0. 745%.
Conclusion:
The method is simple and repeatable and could be used for the quality control of Caulis Akebiae.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP105471985	Calceolarioside B		105471-98-5	Price

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