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[Determination of calycosin-7-O-beta-D-glucopyranoside in Radix Astragali by HPLC]

[Determination of calycosin-7-O-beta-D-glucopyranoside in Radix Astragali by HPLC]

Chun-xia Luo, Ping-chuan Lin, Li-hua Gu, Tao Wu, Da-zheng Wu, Zheng-tao Wang, Zhi-bi Hu

Zhongguo Zhong Yao Za Zhi. 2003 Jul;28(7):603-6.

PMID: 15139100

Abstract:

Objective:
TO establish a RP-HPLC method for the determination of calycosin-7-O-beta-D-glucopyranoside in Radix Astragali, and to analyse the calycosin-7-O-beta-D-glucopyranoside content of ten samples of Radix Astragali, collected from different regions.
Method:
A Polaris C18(250 mm x 4.6 mm, 5 microns) column was used and a mixture of methanol-water (30:70) was used as the mobile phase at a flow rate of 1.0 mL.min-1. The column temperature was 25 degrees C and the UV detection wavelength was 254 nm.
Result:
The calibration curve was in good linearity over the range of 0.0106-2.12 micrograms with the regression equation Y = 3035. 97 X - 14.85(r = 0.9999). The average recovery was 95.8% (n = 5, RSD = 1.3%).
Conclusion:
The method is simple, quick, sensitive and reproducible. In all of the samples, the calycosin-7-O-beta-D-glucopyranoside contents differ markedly.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP20633674	Calycosin 7-O-β-D-glucopyranoside		20633-67-4	Price

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