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Determination of Catecholamines and Related Compounds by Capillary Electrophoresis With Postcolumn Terbium Complexation and Sensitized Luminescence Detection

Determination of Catecholamines and Related Compounds by Capillary Electrophoresis With Postcolumn Terbium Complexation and Sensitized Luminescence Detection

R Zhu, W T Kok

Anal Chem. 1997 Oct 1;69(19):4010-6.

PMID: 9322438

Abstract:

A method has been developed for the determination of catecholamines and related compounds (DA, E, NE, DOPA, DOPAC, DOPAG) in urine samples. The compounds of interest were separated by capillary electrophoresis with a borate buffer as background electrolyte. Detection was based on the sensitized luminescence of terbium ions. Using a homemade postcolumn reactor, a basic solution containing a stoichiometric mixture of terbium(III) chloride and EDTA was added postcolumn to the separation buffer. The ternary catechol-EDTA-terbium complexes formed in the reaction mixture showed a strong luminescence with excitation and emission maxima at 300 and 545 nm, respectively. By optimization of the experimental conditions, zone broadening could be restrained and plate numbers up to 130,000 appeared to be possible. Detection limits found were in the order of 10(-7) mol L-1. The catecholic compounds could be determined in urine samples after a standard cleanup and preconcentration procedure.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP10042883	Terbium(III) chloride		10042-88-3	Price

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