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Determination of Chlorzoxazone and 6-hydroxychlorzoxazone in Human Plasma and Urine by High-Performance Liquid Chromatography

Determination of Chlorzoxazone and 6-hydroxychlorzoxazone in Human Plasma and Urine by High-Performance Liquid Chromatography

R F Frye, D D Stiff

J Chromatogr B Biomed Appl. 1996 Nov 15;686(2):291-6.

PMID: 8971612

Abstract:

A sensitive and reproducible method is described for the determination of the cytochrome P450 enzyme 2E1 substrate chlorzoxazone and its primary metabolite 6-hydroxychlorzoxazone in human plasma and urine. Plasma or diluted urine were acidified, incubated with beta-glucuronidase and then were extracted with diethyl ether. Separation of the analytes was achieved on a C18 column with UV detection set at 283 nm. Excellent linearity was observed over the concentration ranges of 100-3000 ng/ml and 4-400 micrograms/ml in plasma and urine, respectively. The intra-assay variability was < or = 5.1% and the inter-assay variability was < or = 8.2% for each compound in each matrix. The method presented is applicable to pharmacokinetic and pharmacogenetic studies utilizing chlorzoxazone.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP1750454	6-Hydroxychlorzoxazone		1750-45-4	Price

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