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Determination of Chlorzoxazone and 6-hydroxychlorzoxazone in Plasma by Gas Chromatography--Mass Spectrometry

Determination of Chlorzoxazone and 6-hydroxychlorzoxazone in Plasma by Gas Chromatography--Mass Spectrometry

C B Eap, C Schnyder, L Savary

J Chromatogr B Biomed Sci Appl. 1998 Jan 23;705(1):139-44.

PMID: 9498681

Abstract:

A gas chromatographic-mass spectrometric method is presented which allows the determination of chlorzoxazone and 6-hydroxychlorzoxazone after derivatization with the reagent N-tert.-butyldimethylsilyl-N-methyltrifluoroacetamide. No interference was observed from endogenous compounds following the extraction of plasma samples from six different human subjects. The standard curves were linear over a working range of 20 to 4000 ng/ml and of 20 to 1000 ng/ml for chlorzoxazone and 6-hydroxychlorzoxazone, respectively. Recoveries ranged from 65 to 97% for the two compounds and intra- and inter-day coefficients of variation were always less than 9%. The limit of quantitation of the method was found to be 5 ng/ml for the two compounds, hence allowing its use for single low dose pharmacokinetics.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP1750454	6-Hydroxychlorzoxazone		1750-45-4	Price

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