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[Determination of Cyclamate in Complex Matrix Using HPLC After Column Derivatization With 4-fluoro-7-nitrobenzofurazan]

[Determination of Cyclamate in Complex Matrix Using HPLC After Column Derivatization With 4-fluoro-7-nitrobenzofurazan]

M Hauck, H Köbler

Z Lebensm Unters Forsch. Oct-Nov 1990;191(4-5):322-4.

PMID: 2293522

Abstract:

A method for the analysis of cyclamate in complex foodstuffs has been developed. This method is applicable in strongly coloured and protein-rich foodstuffs. The quantitative determination depends on oxidation of cyclamate to cyclohexylamine and derivatisation with 4-fluoro-7-nitrobenzofuran (NBD-F). The derivatives are analysed by HPLC on a C18: reversed-phase column, their minimal stability being 12 h. There are two possible methods of detection: (a) absorbance at 485 nm and (b) fluorescence with excitation at 485 nm and emission at 530 nm. The detection limit of cyclamate is 5 mg/kg foodstuff, with fluorescence detection 0.4 mg/kg. The recoveries are in the range of 88% to 104%.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP29270562	4-Fluoro-7-nitrobenzofurazan		29270-56-2	Price

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