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[Determination of Five Kinds of Trace Ginkgolic Acids in Diterpene Ginkgolides Meglumine Injection Materials by LC-MS/MS]

[Determination of Five Kinds of Trace Ginkgolic Acids in Diterpene Ginkgolides Meglumine Injection Materials by LC-MS/MS]

Hai-hong Si, Yan-jing Li, Jia Xue, Wen-zhe Huang, Zhen-zhong Wang, Wei Xiao

Zhongguo Zhong Yao Za Zhi. 2015 Jul;40(14):2832-6.

PMID: 26666035

Abstract:

To develop a LC-MS/MS method for the determination of five kinds of trace ginkgolic acids in diterpene ginkgolides meglumine injection materials, the column was Agilent ZORBAX Eclipse plus C18 (3.0 mm x 50 mm, 1.8 µm), and the mobile phase consisted of methanol-water (containing 0.2% formic acid) (95:5) at a flow rate of 0.5 mL · min(-1). The multiple reaction ion monitoring (MRM) with an ESI interface in the negative ion mode was selected. The results showed that the linear ranges of five kinds of ginkgolic acids were in the range of 0.2-36.0 µg · L(-1) (r ≥ 0.999 5). The lowest limit of quantification (LOQ) of ginkgo acid C13: 0, C15:1, C17:2, C15:0 and C17:1 were 0.18, 0.18, 0.21, 0.10 and 0.20 µg · L(-1), respectively. The average recovery was between 73.28% and 87.56%, and the average content of total ginkgolic acids in three batches of samples was in the range of 0.023-0.028 µg · g(-1), which was much lower than 2 µg · g(-1) prescribed in drug registration standards. This method is simple and rapid with high sensitivity, which can be used for the determination of five kinds of trace ginkgolic acids in diterpene ginkgolides meglumine injection materials.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP20261385	Ginkgolic acid C13:0		20261-38-5	Price

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