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Determination of Glucosinolates in Traditional Chinese Herbs by High-Performance Liquid Chromatography and Electrospray Ionization Mass Spectrometry

Determination of Glucosinolates in Traditional Chinese Herbs by High-Performance Liquid Chromatography and Electrospray Ionization Mass Spectrometry

Kim-Chung Lee, Man-Wai Cheuk, Wan Chan, Albert Wai-Ming Lee, Zhong-Zhen Zhao, Zhi-Hong Jiang, Zongwei Cai

Anal Bioanal Chem. 2006 Dec;386(7-8):2225-32.

PMID: 17086388

Abstract:

A reversed-phase HPLC method has been developed for determination of twelve intact glucosinolates--glucoiberin, glucocheirolin, progoitrin, sinigrin, epiprogoitrin, glucoraphenin, sinalbin, gluconapin, glucosibarin, glucotropaeolin, glucoerucin, and gluconasturtiin--in ten traditional Chinese plants. The samples were extracted with methanol and the extracts were cleaned on an activated Florisil column. A mobile phase gradient prepared from methanol and 30 mmol L(-1) ammonium acetate at pH 5.0 enabled baseline separation of the glucosinolates. Glucosinolate detection was confirmed by quadrupole time-of-flight tandem mass spectrometric analysis in negative-ionization mode. Detection limits ranged from 0.06 to 0.36 microg g(-1) when 5 g of dried plant was analyzed. Recoveries of the glucosinolates were better than 85% and precision (relative standard derivation, n = 3) ranged from 5.3 to 14.6%. Analysis of the glucosinolates provided scientific evidence enabling differentiation of three pairs of easily confused plants.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP155450310	Glucosibarin		155450-31-0	Price

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