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[Determination of Histamine by a Rapid Fluorescamine-UPLC Method]

Yuuki Murakami, Natsuki Nakai, Naoko Ando, Akiko Okayama

Shokuhin Eiseigaku Zasshi. 2018;59(3):121-125.

PMID: 30033988

Abstract:

We have developed a rapid analysis method for determination of histamine using ultra-performance liquid chromatography and fluorescamine derivatization. Histamine was extracted from foods with trichloroacetic acid after homogenization. The supernatant obtained by centrifugation was filtered and derivatized without solid-phase extraction. The mobile phase was 10 mM phosphate-acetonitrile (75 : 25) without ion-pairing. Recovery tests of histamine from fishes, seasoning and fish products gave acceptable recovery (95.8-117.7%). This method should be useful for rapid evaluation of food poisoning outbreaks.

Chemicals Related in the Paper:

Catalog Number Product Name Structure CAS Number Price
AP38183129 Fluorescamine Fluorescamine 38183-12-9 Price
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