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Determination of L-sesamin and L-asarinin in Zanthoxylum(Roxb.) DC. By High Performance Liquid Chromatography

Determination of L-sesamin and L-asarinin in Zanthoxylum(Roxb.) DC. By High Performance Liquid Chromatography

Shou-Yao Zhang, Ben-Jie Zhou, Yan Wang

Di Yi Jun Yi Da Xue Xue Bao. 2002 Jul;22(7):654-5.

PMID: 12376305

Abstract:

Objective:
To establish a method for determining the content of L-sesamin and L-asarinin indifferent parts of Zanthoxylum(Roxb.)DC.
Method:
High-performance liquid chromatography (HPLC) was utilized for this purpose with the column of HYPERSIL BDS C18 and the mobile phase containing acetonitrile and water (50:50), the detection wavelength being 287 nm.
Results:
The average recovery rates for L-sesamin and L-asarinin were 100.13% and 1.61% and their relative standard deviation (RSD) were 101.24% and 1.46% respectively.
Conclusion:
The method can be used for the quality control of Zanthoxylum(Roxb.)DC, and the roots of this herb contains more L-sesamin and L-asarinin than its other parts do.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP133040	(-)-Asarinin		133-04-0	Price

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