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[Determination of Levamisole Residue in Animal Livers by Two Liquid-Liquid Extraction Steps-Gas Chromatography-Mass Spectrometry]

[Determination of Levamisole Residue in Animal Livers by Two Liquid-Liquid Extraction Steps-Gas Chromatography-Mass Spectrometry]

Jing Xu, Shanshan Xiao, Weifeng Dong, Kai Sui, Jijuan Cao, Wenting Diao, Jing Zhang

Se Pu. 2012 Sep;30(9):922-5.

PMID: 23285974

Abstract:

A novel method wasdeveloped for the determination oflevamisole in animal livers by liquid-liquid extraction and gas chromatography-mass spectrometry. Levamisole hydrochloride was transferred to levamisole in alkalinesolution, andthen extracted intoethyl acetate. Two liquid-liquid extraction steps were carried out with HCI aqueous solution and potassium hydroxide-dichloromethane system in turn, and the liposoluble substances and the water-soluble substances were removed, respectively. The qualitative and quantitative analyses of levamisole were achieved by gas chromatography-mass spectrometry (GC-MS) system. The characteristic fragments m/z 148, 176 and 204 were selected and m/z 204 was used as the quantitative ion. A good linear relationship was obtained between the peak area and the mass concentration of levamisole from 0.25 mg/L to 3.0 mg/L with the correlation coefficient of 0.999. The limit of quantitation (LOQ) for levamisole was 5 microg/kg, which was much lower than the international maximum residue limit. The average recoveries of levamisole spiked in chicken liver, duck liver, rabbit liver and pig liver samples at three spiked levels ranged from 76% to 106% with the relative standard deviations less than 9%. The method is simple and stable without tedious sample preparation, and suitable for the determination of levamisole in animal livers.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP16595805-B	(−)-Levamisole hydrochloride solution		16595-80-5	Price

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