Home
Resources
Publications
Determination of Liposome-Buffer Distribution Coefficients of Charged Drugs by Capillary Electrophoresis Frontal Analysis

Determination of Liposome-Buffer Distribution Coefficients of Charged Drugs by Capillary Electrophoresis Frontal Analysis

Ulrik Franzen, Lene Jorgensen, Claus Larsen, Niels H H Heegaard, Jesper Østergaard

Electrophoresis. 2009 Aug;30(15):2711-9.

PMID: 19621377

Abstract:

The potential of using CE frontal analysis (CE-FA) to study the interactions between a range of charged low molecular weight drug substances and liposomes was evaluated. The liposomes used were net negatively charged and consisted of 2-oleoyl-1-palmitoyl-sn-glycero-3-phosphocholine and 1,2-dipalmitoyl-sn-glycero-3-phosphate monosodium salt in a ratio of 80/20 mol%. Apparent distribution coefficients (D(mem)), defined as the molar concentration of drug substance in the membrane phase divided by the molar concentration of drug substance in the aqueous phase, were successfully determined for six positively and eight negatively charged drug substances with log D(mem) ranging from 1.35 to 3.63. The extent of liposome-buffer distribution was found to be dependent on the drug concentration. The results obtained with the developed CE-FA method were in good agreement with results obtained by equilibrium dialysis. Furthermore, the CE-FA method was faster, less labor intensive and required smaller sample volumes (approximately 50 microL) compared with equilibrium dialysis. Thus, CE-FA is an efficient and useful tool for the characterization of interactions between liposomes and low molecular weight drug substances.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP169051609	1,2-Dipalmitoyl-sn-glycero-3-phosphate monosodium salt		169051-60-9	Price

As a specialist in analytical chemical Products, Alfa Chemistry offers consumers a wealth of raw materials and a full range of technologies and services.

QUICK LINKS

HEADQUARTERS

Tel:

Fax:

Email: