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Determination of Methenamine, Methenamine Mandelate and Methenamine Hippurate in Pharmaceutical Preparations Using Ion-Exchange HPLC

Determination of Methenamine, Methenamine Mandelate and Methenamine Hippurate in Pharmaceutical Preparations Using Ion-Exchange HPLC

Chiravi Pavitrapok, David A Williams

J Pharm Biomed Anal. 2006 Mar 18;40(5):1243-8.

PMID: 16298099

Abstract:

An ion-exchange column high-performance liquid chromatography (HPLC) method has been developed for the determination of methenamine in methenamine and methenamine hippurate pharmaceutical preparations. The HPLC method uses a Zorbax SCX-300 column with acetonitrile-0.1M sodium perchlorate monohydrate (pH 5.8) (70:30, v/v) as the mobile phase at the flow rate of 1 mL/min. UV-detection was at 212 nm. The linear concentration plots for methenamine were linear over the concentration range of 0.25-50mM for methenamine and methenamine mandelate standards. The intra-day RSD precision was <1.25%, and for inter-day, <1.85%. The peaks for mandelic acid, hippuric acid and the other ingredients from placebo tablets do not interfere with the analysis for methenamine. The accuracy of this method was shown to be 99-101% by measuring the recovery of methenamine from spiked placebo tablets. The assay of methenamine from methenamine hippurate tablets and from a urinary antiseptic tablet containing methenamine were in the range of 98-102%. This HPLC method is a fast, simple and straightforward method for the analysis of methenamine in pharmaceutical preparations.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP587235	Methenamine mandelate		587-23-5	Price
AP7791073	Sodium perchlorate monohydrate		7791-07-3	Price

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