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[Determination of Nine Steroidal Saponins in Paris Polyphylla From Different Areas of Guizhou Province by HPLC]

[Determination of Nine Steroidal Saponins in Paris Polyphylla From Different Areas of Guizhou Province by HPLC]

Yuyong Liang, Zhen Liu, Wenyuan Gao, Shuli Man

Zhongguo Zhong Yao Za Zhi. 2012 Aug;37(15):2309-12.

PMID: 23189740

Abstract:

Objective:
To compare steroidal saponins-main active ingredients of Paris polyphylla from different areas in Guizhou province, in order to provided basis for further proving Guizhou province to be the planting base of P. polyphylla.
Method:
The contents of nine steroidal saponins, namely paris-VII, (25R)-5-en-spirost-3beta,17alpha-diol-3-O-alpha-L-rhamnopyranosyl-(1-->2) [alpha-L-rhamnopyranosyl(1-->4)]-beta-D-glycopyanoside (PGRR), paris-H, paris-VI, paris-II , paris-III, gracillin, paris-I and paris-V of P. polyphylla from eight areas of Guizhou province were determined by HPLC-UV.
Result:
Five steroidal saponins-paris-VII, PGRR, paris-H, paris-VI and gracillin were detected in all the drugs, which provided basis for distinguishing P. polyphylla. P. polyphylla from Dushan showed the highest content of steroidal saponins (9.62%), followed by Tongren (6.39%), and the lowest was Zunyi (0.99%).
Conclusion:
Saponins from different areas of Guizhou province show significant difference in variety and content, therefore Dushan is suitable for planting P. polyphylla.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP68124049	Paris VII		68124-04-9	Price

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