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Determination of Pseudouridine in Human Urine and Serum by High-Performance Liquid Chromatography With Post-Column Fluorescence Derivatization

Y Umegae, H Nohta, Y Ohkura

J Chromatogr. 1990 Aug 31;515:495-501.

PMID: 2283375

Abstract:

A selective and sensitive method for the determination of pseudouridine in human urine and serum is described. The method is based on high-performance liquid chromatography with post-column fluorescence derivatization. Pseudouridine and 5-fluorouridine (internal standard) in a 10-fold diluted urine sample or a deproteinized serum sample are separated on a reversed-phase column (TSK gel ODS-80) with isocratic elution and successively subjected to derivatization involving periodate oxidation followed by fluorescence reaction with meso-1,2-bis(4-methoxyphenyl)ethylenediamine. The detection limit for pseudouridine is 4 pmol in a 100-microliters injection volume.

Chemicals Related in the Paper:

Catalog Number Product Name Structure CAS Number Price
AP58520459 meso-1,2-Bis(4-methoxyphenyl)ethylenediamine meso-1,2-Bis(4-methoxyphenyl)ethylenediamine 58520-45-9 Price
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