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Determination of Salvianolic Acid C in Rat Plasma Using Liquid Chromatography-Mass Spectrometry and Its Application to Pharmacokinetic Study

Junke Song, Wen Zhang, Jialin Sun, Xue Zhang, Xiaona Xu, Li Zhang, Zhangying Feng, Guanhua Du

Biomed Chromatogr. 2016 Mar;30(3):376-83.

PMID: 26173992

Abstract:

A sensitive and reliable LC-ESI-MS method for the determination of salvianolic acid C in rat plasma has been developed and validated. Plasma samples were prepared by liquid-liquid extraction with ethyl acetate and separated on a Zorbax SB-C18 column (3.5 µm, 2.1 × 100 mm) at a flow rate of 0.3 mL/min using acetonitrile-water as mobile phase. The detection was carried out by a single quadrupole mass spectrometer with electrospray ionization source and selected ion monitoring mode. Linearity was obtained for salvianolic acid C ranging from 5 to 1000 ng/mL. The intra- and inter-day precisions (RSD, %) didn't exceed 9.96%, and the accuracy (RE, %) were all within ±3.64%. The average recoveries of the analyte and internal standard were >89.13%. Salvianolic acid C was proved to be stable during all sample storage, preparation and analytic procedures. The validated method was successfully applied to pharmacokinetic study after oral and intravenous administration of salvianolic acid C to rats. The absolute oral bioavailability of salvianolic acid C was 0.29 ± 0.05%. This method was further applied to simultaneous determination of salvianolic acid A, salvianolic acid B and salvianolic acid C in rat plasma and showed good practicability.

Chemicals Related in the Paper:

Catalog Number Product Name Structure CAS Number Price
AP115841093 Salvianolic acid C Salvianolic acid C 115841-09-3 Price
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