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[Determination of Seven Monosaccharides in Aloe Polysaccharide Using Ion Chromatography With Pulsed Amperometric Detector]

[Determination of Seven Monosaccharides in Aloe Polysaccharide Using Ion Chromatography With Pulsed Amperometric Detector]

Shengfeng Le, Wei Wang, Yaxuan Wang, Xinying Zhao

Se Pu. 2019 Mar 8;37(3):319-324.

PMID: 30900862

Abstract:

Aloe polysaccharide was extracted by water extraction and ethanol precipitation. It was hydrolyzed by trifluoroacetic acid, eluted by a sodium hydroxide gradient on a Dionex CarboPac PA10 column, and detected using a pulsed amperometric detector. This method was used to determine the fucose, rhamnose, arabinose, galactose, glucose, mannose, and xylose in aloe polysaccharide. The results showed that the correlation coefficients (R2) of all the seven target compounds were more than 0.997 in the linear range, and the limits of detection were between 0.007 mg/L and 0.024 mg/L. The recoveries were in the range 97.5%-102.5% with the relative standard deviations in the range 0.1%-4.8%. The method is simple, rapid, sensitive, and accurate for the determination of the monosaccharide content and the constitution of aloe polysaccharides.

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Catalog Number	Product Name	Structure	CAS Number	Price
AS23124	Galactose standard for IC			Price

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