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Determination of Taurine in Biological Samples by High-Performance Liquid Chromatography Using 4-fluoro-7-nitrobenzofurazan as a Derivatizing Agent

Determination of Taurine in Biological Samples by High-Performance Liquid Chromatography Using 4-fluoro-7-nitrobenzofurazan as a Derivatizing Agent

XiFeng Wang, DeFeng Chi, GuanMin Su, Lin Li, LiHua Shao

Biomed Environ Sci. 2011 Oct;24(5):537-42.

PMID: 22108421

Abstract:

Objective:
A highly sensitive and rapid high-performance liquid chromatography method with pre-column derivatization with 4-fluoro-7-nitrobenzofurazan was developed for determination of taurine in biological samples, including plasma, brain, and liver.
Methods:
The optimum derivatization reaction temperature was 70 °C, and at this temperature the reaction was complete within 3 min. The derivatized taurine was separated using phosphate buffer (0.02 mol/L, pH 6.0):acetonitrile (84:16, v/v) as the mobile phase at a flow rate of 1.0 mL/min, and a column temperature of 25 °C. The taurine derivatives were separated within 20 min (tR:14.5 min) and fluorometrically detected at 530 nm with excitation at 470 nm.
Results:
The intra- and the inter-day coefficients of variation for the method were 5.3% and 7.7%, respectively. The calibration curve was linear from 0.1 μmol/L to 30.0 μmol/L with a correlation coefficient of 0.9995.
Conclusion:
This method can be used to determine the taurine contents in plasma, brain, and liver from normal rats and human plasma.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP29270562	4-Fluoro-7-nitrobenzofurazan		29270-56-2	Price

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